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SI-PB-RI [id:]

Method: Ecological status assessment system for rivers using phytobenthos [Vrednotenje ekološkega stanja rek s fitobentosom]

1. General information

1.01 GIG: Alpine, Eastern Continental, Mediterranean
Relevant intercalibration types:
Alpine: R-A, Eastern Continental: R-E4 ,R-EX5, R-EX6, Mediterranean: R-M1, R-M2, R-M5
1.02 Category: Rivers
1.03 BQE: Benthic Diatoms
1.04 Country: Slovenia
1.05 Specification: none
1.06 Method name: Ecological status assessment system for rivers using phytobenthos
1.07 Original name: Vrednotenje ekološkega stanja rek s fitobentosom
1.08 Status: Method is/will be used in First RBMP (2009)
1.09 Detected pressure(s):
Eutrophication, Pollution by organic matter Pressure-impact-relationship: No, pressure-impact relationship has not been tested.
1.10 Internet reference:
1.11 Pertinent literature of mandatory character:
Uradni list Republike Slovenije stran (pp) 832, st. (no) 10, 9.2.2009.
1.12 Scientific literature: n.a.
1.13 Method developed by: Gorazd Kosi
Email of developer:
Institute of developer: Nationale Institute of Biology
1.14 Method reported by: Gorazd Kosi
Email of person reporting the method:
Email of institute reporting the method: National Institute of Biology
1.15 Comments: none

2. Data acquisition

Field sampling/surveying

2.01 Sampling/Survey guidelines:
Kosi, G., M. Sisko, N. Smolar-Zvanut, D. Vrhovsek, A. Krivograd-Klemencic, 2005. Priprava metodologije vzorcenja ter laboratorijske obdelave vzorcev alg (fitobentosa) za dolocanje ekoloskega stanja vodotokov v Sloveniji in obdelava 45 vzorcev alg. Nacionalni institut za biologijo, 72 str.
2.02 Short description:
Brushing and splashing of different substrates collected from different habitats. Organisms from all substrates represent a sample.
2.03 Method to select the sampling/survey site or area: Expert knowledge Other method to select the sampling/survey site or area: Expert knowledge (e.g. sites most representative of water body)
2.04 Sampling/survey device: Brush
2.05 Specification: Brush
2.06 Sampled/surveyed habitat:
Sampled habitat: All available habitats per site (Multi-habitat)
2.07 Sampled/surveyed zones in areas with tidal influence: not relevant
2.08 Sampling/survey month(s):
Small to large rivers: June - September, very large rivers: December - February
2.09 Number of sampling/survey occasions (in time) to classify site or area: One occasion per sampling season
2.10 Number of spatial replicates per sampling/survey occasion to classify site or area: Several
2.11 Total sampled/surveyed area or volume or total sampling duration to classify site or area:

Sample processing

2.12 Minimum size of organisms sampled and processed: n.a.
2.13 Sample treatment:
500 valves per sample are counted.
Sample is divided (sub-sampling) and organisms of a sub-sample are identified.
2.14 Level of taxonomical identification:
Level: Species/species groups
Specification of level of determination: n.a.
2.15 Record of abundance:
Determination of abundance: Relative abundance
Abundance is related to: n.a.
Unit of the record of abundance: Number of individuals of 500 counted valves.
2.16 Quantification of biomass: n.a.
2.17 Other biological data: none
2.18 Special cases, exceptions, additions: none
2.19 Comments: none

3. Data evaluation


3.01 List of biological metrics:
Saprobic index (SI = Sum of (Indicator Taxa Abundance x Saprobic value x Indicator weight) / Indicator Taxa Abundance x Indicator weight), Trophic index (TI = Sum of (Indicator Taxa Abundance x Trophic value x Indicator weight) / Indicator Taxa Abundance x Indicator weight)
3.02 Does the metric selection differ between types of water bodies: No
3.03 Combination rule for multi-metrics: Worst metric score
3.04 From which biological data are the metrics calculated:
List of biological metrics: Data from single sampling/survey occasion in time

Reference conditions

3.05 Scope of reference conditions: Surface water type-specific
3.06 Key source(s) to derive reference conditions:
Scope of reference conditions:
Existing near-natural reference sites, Expert knowledge, Modelling (extrapolating model results)
3.07 Reference site characterisation:
Number of sites: Differ among types (up to 10)
Geographical coverage: Alps and Dinarids and Pannonian lowland
Location of sites: n.a.
Data time period: 1998-2007
The criteria for the selection of the potential reference sites in the rivers include hydromorphological and physico-chemical conditions of the site, riparian vegetation, floodplain and land use properties, saprobic index values, and some pressures' presence. Potential reference sites were defined without considering the criteria of biotic pressures that includes allochthonous species and fishery management.
3.08 Reference community description: n.a.
3.09 Results expressed as EQR: Yes

Boundary setting

3.10 Setting of ecological status boundaries:
Equidistant division of the EQR gradient
High-good boundary derived from metric variability at near-natural reference sites
3.11 Boundary setting procedure: n.a.
3.12 "Good status" community: n.a.


3.13 Consideration of uncertainty: Yes
Specification of uncertainty consideration:
We tested the influence of number of samples on variability of EQR values at individual sampling sites.
3.14 Comments: none

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