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SI-PB-LA [id:]

Method: Ecological status assessment system for lakes using phytobenthos [Vrednotenje ekološkega stanja jezer s fitobentosom]

1. General information

1.01 GIG: Alpine
Relevant intercalibration types: Alpine GIG, L-AL3
1.02 Category: Lakes
1.03 BQE: Benthic Diatoms
1.04 Country: Slovenia
1.05 Specification: none
1.06 Method name: Ecological status assessment system for lakes using phytobenthos
1.07 Original name: Vrednotenje ekološkega stanja jezer s fitobentosom
1.08 Status: Method is/will be used in First RBMP (2009)
1.09 Detected pressure(s):
Eutrophication Pressure-impact-relationship: No, pressure-impact relationship has not been tested.
1.10 Internet reference:
1.11 Pertinent literature of mandatory character: n.a.
1.12 Scientific literature: n.a.
1.13 Method developed by: Gorazd Kosi
Email of developer:
Institute of developer: Nationale Institute of Biology
1.14 Method reported by: Gorazd Kosi
Email of person reporting the method:
Email of institute reporting the method: National Institute of Biology
1.15 Comments: none

2. Data acquisition

Field sampling/surveying

2.01 Sampling/Survey guidelines:
Kosi, G. & M. Bricelj, 2006. Metodologija vzorcenja in laboratorijske obdelave fitobentosa v jezerih v skladu z zahtevami vodne direktive (Direktiva 2000/60/ES), Nacionalni institut za biologijo, 11 str.
2.02 Short description:
Brushing and splashing of different substrates collected from different habitats. Organisms from all substrates represent a sample.
2.03 Method to select the sampling/survey site or area: Expert knowledge Other method to select the sampling/survey site or area: Expert knowledge (e.g. sites most representative of water body)
2.04 Sampling/survey device: Brush
2.05 Specification: Brush
2.06 Sampled/surveyed habitat:
Sampled habitat: All available habitats per site (Multi-habitat)
2.07 Sampled/surveyed zones in areas with tidal influence: not relevant
2.08 Sampling/survey month(s): June - September
2.09 Number of sampling/survey occasions (in time) to classify site or area: One occasion per sampling season
2.10 Number of spatial replicates per sampling/survey occasion to classify site or area: 3 samples per lake
2.11 Total sampled/surveyed area or volume or total sampling duration to classify site or area:

Sample processing

2.12 Minimum size of organisms sampled and processed: n.a.
2.13 Sample treatment:
500 valves per sample are counted.
Sample is divided (sub-sampling) and organisms of a sub-sample are identified.
2.14 Level of taxonomical identification:
Level: Species/species groups
Specification of level of determination: n.a.
2.15 Record of abundance:
Determination of abundance: Relative abundance
Abundance is related to: n.a.
Unit of the record of abundance: Number of individuals of 500 counted valves.
2.16 Quantification of biomass: n.a.
2.17 Other biological data: none
2.18 Special cases, exceptions, additions: none
2.19 Comments: none

3. Data evaluation


3.01 List of biological metrics:
Trophic index (TI = Sum of (Indicator Taxa Abundance x Trophic value x Indicator weight) / Indicator Taxa Abundance x Indicator weight)
3.02 Does the metric selection differ between types of water bodies: No
3.03 Combination rule for multi-metrics: Worst metric score
3.04 From which biological data are the metrics calculated:
List of biological metrics: Data from single spatial replicate

Reference conditions

3.05 Scope of reference conditions: Surface water type-specific
3.06 Key source(s) to derive reference conditions:
Scope of reference conditions: Existing near-natural reference sites, Expert knowledge
3.07 Reference site characterisation:
Number of sites: 10 sites in one lake
Geographical coverage: Alps
Location of sites: Bohinjsko jezero
Data time period: 2006-2007
Criteria: n.a.
3.08 Reference community description: n.a.
3.09 Results expressed as EQR: Yes

Boundary setting

3.10 Setting of ecological status boundaries:
Equidistant division of the EQR gradient
High-good boundary derived from metric variability at near-natural reference sites
3.11 Boundary setting procedure: n.a.
3.12 "Good status" community: n.a.


3.13 Consideration of uncertainty: No (to be done)
3.14 Comments: none

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Online: [date: 2021/05/18]
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