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BG-DI-RI [id:]

Method: Sampling, preservation and pretreatment of benthic diatoms [?????????????, ???????????? ? ????????????? ????????? ?? ????? ?? ???????? (?????????) ?????????]

1. General information

1.01 GIG: Eastern Continental
Relevant intercalibration types: n.a.
1.02 Category: Rivers
1.03 BQE: Benthic Diatoms
1.04 Country: Bulgaria
1.05 Specification: n.a.
1.06 Method name: Sampling, preservation and pretreatment of benthic diatoms
1.07 Original name:
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1.08 Status: Method is/will be used in Second RBMP (2015)
1.09 Detected pressure(s):
Eutrophication, Hydromorphological degradation, Pollution by organic matter Specification of pressure-impact-relationship: n.a.
Pressure-impact-relationship: No, pressure-impact relationship has not been tested.
1.10 Internet reference: n.a.
1.11 Pertinent literature of mandatory character: n.a.
1.12 Scientific literature: n.a.
1.13 Method developed by: Plamen Ivanov
Email of developer:
Institute of developer: Sofia University "St. Kliment Ohridski", Faculty of Biology
1.14 Method reported by: Valeriya Gyosheva
Email of person reporting the method:;
Email of institute reporting the method: Executive Environmental Agency
1.15 Comments: none

2. Data acquisition

Field sampling/surveying

2.01 Sampling/Survey guidelines:
EN 13946: 2003. Water quality - Guidance standard for the routine sampling and pretreatment of benthic diatoms from rivers
2.02 Short description:
Samples are collected acc. to EN 13946:2003. The most usable bottom substrata for diatom monitoring are stones, gravel, rocks, concrete (epilithic diatom communities). Samples are taken at riffle sites within the monitoring stations. If it is possible, sampling should cover both river banks, shady and sunny sites, and various microhabitats. The upper and side parts of 5-8 submerged stones / walls of concrete canals or other hard substratum are scraped by hard brush. Collected composite sample with about 50 ml volume is poured into a container and it is preserved by 4% formaldehyde.
2.03 Method to select the sampling/survey site or area: Stratified sampling/surveying
2.04 Sampling/survey device: Brush
2.05 Specification: none
2.06 Sampled/surveyed habitat:
Specification of sampled habitat: Stones, gravel, rocks, concrete - epilitic diatom communities
Sampled habitat: Single habitat(s)
2.07 Sampled/surveyed zones in areas with tidal influence: not relevant
2.08 Sampling/survey month(s): June - September
2.09 Number of sampling/survey occasions (in time) to classify site or area: once per year in summer
2.10 Number of spatial replicates per sampling/survey occasion to classify site or area: n.a.
2.11 Total sampled/surveyed area or volume or total sampling duration to classify site or area:

Sample processing

2.12 Minimum size of organisms sampled and processed: none
2.13 Sample treatment:
Organisms of the complete sample are identified.
2.14 Level of taxonomical identification:
Level: Species/species groups
2.15 Record of abundance:
Determination of abundance: Percent coverage
Abundance is related to: n.a.
Unit of the record of abundance: n.a.
2.16 Quantification of biomass: n.a.
2.17 Other biological data:
2.18 Special cases, exceptions, additions: none
2.19 Comments: none

3. Data evaluation


3.01 List of biological metrics:
IPS (Diatoms), Abundance of Cladophora sp., Presence and abundance of filamentous bacteria
3.02 Does the metric selection differ between types of water bodies: Yes
3.03 Combination rule for multi-metrics: n.a.
3.04 From which biological data are the metrics calculated:
List of biological metrics: Data from single sampling/survey occasion in time

Reference conditions

3.05 Scope of reference conditions: Surface water type-specific
3.06 Key source(s) to derive reference conditions:
Scope of reference conditions: Expert knowledge, Existing near-natural reference sites
3.07 Reference site characterisation:
Number of sites: 21 sites in the Danube plain
Geographical coverage: n.a.
Location of sites: n.a.
Data time period: Main data are from 2009 (June to October)
Criteria: n.a.
3.08 Reference community description: n.a.
3.09 Results expressed as EQR: Yes

Boundary setting

3.10 Setting of ecological status boundaries: n.a.
3.11 Boundary setting procedure: n.a.
3.12 "Good status" community: n.a.


3.13 Consideration of uncertainty: n.a.
Specification of uncertainty consideration: n.a.
3.14 Comments: none

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