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ES-MA-LA [id:]

Method: Aquatic Flora Spanish Assessment Method [n.a.]

1. General information

1.01 GIG: Mediterranean
Relevant intercalibration types: n.a.
1.02 Category: Lakes
1.03 BQE: Benthic Diatoms, Macrophytes, Other Phytobenthos
1.04 Country: Spain
1.05 Specification: n.a.
1.06 Method name: Aquatic Flora Spanish Assessment Method
1.07 Original name: n.a.
1.08 Status: Method is/will be used in n.a.
1.09 Detected pressure(s):
Eutrophication, Hydromorphological degradation, Alien species Specification of pressure-impact-relationship: n.a.
Pressure-impact-relationship: No, pressure-impact relationship has not been tested.
1.10 Internet reference: n.a.
1.11 Pertinent literature of mandatory character: n.a.
1.12 Scientific literature: n.a.
1.13 Method developed by: n.a.
Email of developer: n.a.
Institute of developer: n.a.
1.14 Method reported by: Guillermo Martinez
Email of person reporting the method:
Email of institute reporting the method: CEDEX
1.15 Comments: none

2. Data acquisition

Field sampling/surveying

2.01 Sampling/Survey guidelines: n.a.
2.02 Short description: n.a.
2.03 Method to select the sampling/survey site or area: n.a.
2.04 Sampling/survey device: Bathiscope, if not possible, rake and grapnel
2.05 Specification: none
2.06 Sampled/surveyed habitat:
Specification of sampled habitat: n.a.
Sampled habitat: n.a.
2.07 Sampled/surveyed zones in areas with tidal influence: not relevant
2.08 Sampling/survey month(s):
Macrophytes sampling: Different periods have been considered according to the kind of lake: - Lakes deeper than 3 meters: July to September,- Lakes shallower than 3 meters and permanent: May to July, - Lakes shallower than 3 meters and temporal: one month
2.09 Number of sampling/survey occasions (in time) to classify site or area:
Macrophytes sampling: Once or twice per year. Two data per year are proposed in the macrophytes sampling protocol. However, if there is not economic availability to carry out two samplings, it is possible to make only one sampling a year in the date which
2.10 Number of spatial replicates per sampling/survey occasion to classify site or area:
Macrophyte sampling:
Different criteria have been considered for the sampling of hydrophytes and helophytes according to morphometric characteristics of the lakes; Hydrophytes sampling points: - Lakes shallower than 2 m: 10 sampling points: 5 in the hiLSID{60664CAF-AF0D-0004-A300-5C7 I5???0}
2.11 Total sampled/surveyed area or volume or total sampling duration to classify site or area:
Macrophytes sampling: Hydrophytes sampling points:- Lakes smaller than 50 ha: 10 sampling points,- Lakes bigger than 50 ha: 20 sampling points; Helophytes sampling points: - Lakes with a perimeter of less than 1 km: the whole perimeter has to be sampled;

Sample processing

2.12 Minimum size of organisms sampled and processed: n.a.
2.13 Sample treatment:
2.14 Level of taxonomical identification:
Level: Species/species groups
Specification of level of determination:
Macrophytes sampling:
All the groups of species: vascular species, carophytes, bryophytes and filamentosus algae have to be determined to species level;

Phytobenthos sampling:
Diatoms have to be determined to species level
2.15 Record of abundance:
Determination of abundance: n.a.
Abundance is related to: n.a.
Unit of the record of abundance: n.a.
2.16 Quantification of biomass: n.a.
2.17 Other biological data:
2.18 Special cases, exceptions, additions: none
2.19 Comments: none

3. Data evaluation


3.01 List of biological metrics:
Metrics which assess eutrophication pressures: InDia (diatoms Index), Coverage of eutrophication species of macrophytes; Metrics which assess hydromorphological pressures: Presence/absence of hydrophytes, Macrophytes richness, Total coverage of hydrophytes, Total coverage of helophytes, Total coverage of macrophytes (hydrophytes+helophytes); Metrics which assess introduction of exotic macrophytes species: Coverage of exotic macrophytes species
3.02 Does the metric selection differ between types of water bodies: According to the lake type, some specific metrics are not used.
3.03 Combination rule for multi-metrics: Average metric score; Worst quality class
Other rules:
Metrics which assess the same pressure: average of the metrics normalized EQRs values, Metrics which assess different pressures: worst value of the metrics normalized EQRs values.
3.04 From which biological data are the metrics calculated:
List of biological metrics: n.a.

Reference conditions

3.05 Scope of reference conditions: Site-specific
3.06 Key source(s) to derive reference conditions:
Scope of reference conditions: n.a.
3.07 Reference site characterisation:
Number of sites:
Ca. 70 reference sites for 30 types considered in the Spanish lake typology. Nevertheless, for some lake types reference sites have to be considered as benchmark sites (best ecologcial status within the type).
Geographical coverage: n.a.
Location of sites: n.a.
Data time period: n.a.
Pressure indicators: land use, hm mod, wastewater, alien species, recreation, status of associated groundwater body and surface water body. Ecoloogical indicators: trophic status acc to TP
3.08 Reference community description: n.a.
3.09 Results expressed as EQR: n.a.

Boundary setting

3.10 Setting of ecological status boundaries:
High-good boundary derived from metric variability at near-natural reference sites
3.11 Boundary setting procedure:
Percentiles of the data distribution on reference sites, expert judgment
3.12 "Good status" community:
There are descriptions of macrophytes characteristic species of each lake type.


3.13 Consideration of uncertainty: n.a.
Specification of uncertainty consideration: n.a.
3.14 Comments: none

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