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IBD [id:]

Method: Biological Diatom Index [Indice Biologique Diatomées]

1. General information

1.01 GIG: Central-Baltic
Relevant intercalibration types: n.a.
1.02 Category: Rivers
1.03 BQE: Benthic Diatoms
1.04 Country: Luxembourg
1.05 Specification:
Only for the rivers making the borders of Luxembourg, sometimes the assessment method of the neighbouring country is applied.
1.06 Method name: Biological Diatom Index
1.07 Original name: Indice Biologique Diatomées
1.08 Status: Method is/will be used in First RBMP (2009)
1.09 Detected pressure(s):
Eutrophication Specification of pressure-impact-relationship:
The assessment methods have not been tested especially for Luxembourg, but the indices we are using are French or international standardized methods.
Pressure-impact-relationship: No, pressure-impact relationship has not been tested.
1.10 Internet reference: n.a.
1.11 Pertinent literature of mandatory character:
NF T90-354 Décembre 2007, Qualité de l'eau - Détermination de l'Indice Biologique Diatomées (IBD), Indice de classement
1.12 Scientific literature:
1.13 Method developed by: n.a.
Email of developer: n.a.
Institute of developer: n.a.
1.14 Method reported by: Nora Welschbillig
Email of person reporting the method:
Email of institute reporting the method:
Administration de la Gestion de l'Eau - Ministère de l'Intérieur et á la Grande Région

2. Data acquisition

Field sampling/surveying

2.01 Sampling/Survey guidelines:
NF T90-354 Décembre 2007, Qualite de l'eau - Détermination de l'Indice Biologique Diatomees (IBD), Indice de classement : T90-354
2.02 Short description: 5 to 10 stones in the rover flow
2.03 Method to select the sampling/survey site or area: Expert knowledge Other method to select the sampling/survey site or area: Sites most representative of water body
2.04 Sampling/survey device: Brush
2.05 Specification: scraper or brush
2.06 Sampled/surveyed habitat:
Sampled habitat: Single habitat(s)
2.07 Sampled/surveyed zones in areas with tidal influence: not relevant
2.08 Sampling/survey month(s): May to October
2.09 Number of sampling/survey occasions (in time) to classify site or area: One occasion per sampling season
2.10 Number of spatial replicates per sampling/survey occasion to classify site or area: 1
2.11 Total sampled/surveyed area or volume or total sampling duration to classify site or area:
Minimum size of survey = 100 cm2

Sample processing

2.12 Minimum size of organisms sampled and processed: About 100 cm2 biofilm sampled on stones
2.13 Sample treatment:

Sample is divided (sub-sampling) and organisms of a sub-sample are identified.
2.14 Level of taxonomical identification:
Level: Species/species groups
Specification of level of determination: n.a.
2.15 Record of abundance:
Determination of abundance: Individual counts, Relative abundance
Abundance is related to: Area
Unit of the record of abundance: Number of individuals per sampled area
2.16 Quantification of biomass: n.a.
2.17 Other biological data: none
2.18 Special cases, exceptions, additions: none
2.19 Comments: none

3. Data evaluation


3.01 List of biological metrics:
Relative abundance of key taxa (Ax), with pollution sensitivity (Pxi) and valence values (Vx) (=F(i)=B = 1x F(1) + 2x F(2) + 3x F(3) + 4x F(4) + 5x F(5) + 6x F(6) + 7x F(7)
B is transformed into a /20 note.
3.02 Does the metric selection differ between types of water bodies: n.a.
3.03 Combination rule for multi-metrics: n.a.
3.04 From which biological data are the metrics calculated:
List of biological metrics: Data from single sampling/survey occasion in time

Reference conditions

3.05 Scope of reference conditions: Surface water type-specific
3.06 Key source(s) to derive reference conditions:
Scope of reference conditions:
Existing near-natural reference sites, Expert knowledge, Modelling (extrapolating model results)
3.07 Reference site characterisation:
Number of sites: n.a.
Geographical coverage: n.a.
Location of sites: n.a.
Data time period: n.a.
Criteria: n.a.
3.08 Reference community description: n.a.
3.09 Results expressed as EQR: n.a.

Boundary setting

3.10 Setting of ecological status boundaries: n.a.
3.11 Boundary setting procedure: n.a.
3.12 "Good status" community: n.a.


3.13 Consideration of uncertainty: n.a.
Specification of uncertainty consideration: n.a.
We are applying the indices and methods developed mostly in France and are not involved in the development and adjustments of these methods. Therefore, we are not able to give further informations on the method-related questions.

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Online: [date: 2019/05/24]
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