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Method: Lakes and Reservoirs Biological Quality Assessment Method - Phytoplankton [Método de Avaliação da Qualidade Biológica de Lagos e Albufeiras - Fitoplâncton]

1. General information

1.01 GIG: Mediterranean
Relevant intercalibration types:
Siliceous reservoirs "Wet" Areas. Several attempts were made to intercalibrate the three types of natural lakes from Azores in the first and second phase of the intercalibration exercise. The latest findings, after attempting to intercalibrate with the vo
1.02 Category: Lakes
1.03 BQE: Phytoplankton
1.04 Country: Portugal
1.05 Specification:
Not applicable to run-of-river reservoirs. Assessment Method Metrics are being tested in Southern Reservoirs but sampling method is the same. Assessment Method Metrics are being tested in natural Lakes in Azores but sampling method is the same.
1.06 Method name:
Lakes and Reservoirs Biological Quality Assessment Method - Phytoplankton
1.07 Original name:
Método de Avaliação da Qualidade Biológica de Lagos e Albufeiras - Fitoplâncton
1.08 Status: Method is/will be used in First RBMP (2009)
1.09 Detected pressure(s):
Eutrophication Specification of pressure-impact-relationship:
Assessment metrics were selected during MedGIG Intercalibration Exercise. The selection was based on a project using a set of 33 Spanish reservoirs (see De Hoyos et al. 2005) according to best Pearson correlation with Total P (r>0.7). Currently the method is being tested and adapted to the natural lakes of the Azores Archipelago.
Pressure-impact-relationship:
Yes, with quantitative data (e.g. against range of sites reflecting continuous gradient of pressure).
1.10 Internet reference:
http://dqa.inag.pt/dqa2002/port/docs_apoio/nacionais
html; http://www.azores.gov.pt/Gra/sram-drotrh/menus/principal/Publicações/;
http://www.azores.gov.pt/Gra/sram-drotrh/conteudos/projectos/2009/Abril/RSCRHAçores.htm?lang=pt&area=ct
1.11 Pertinent literature of mandatory character:
National sampling protocol: Inag, I.P., 2009. Manual para a Avaliacao da Qualidade Biológica da água em Lagos e Albufeiras segundo a Directiva Quadro da água - Protocolo de Amostragem e análise para o Fitoplancton. Ministério do Ambiente, do Ordenamento do Território e do Desenvolvimento Regional. Instituto da água, I. P. (available online). National Ecological Status Classification Guidelines: Inag, I.P., 2009. Critérios para a Classificação do Estado das Massas de água Superficiais ? Rios e Albufeiras. Ministério do Ambiente, do Ordenamento do Território e do Desenvolvimento Regional. Instituto da água, I. P. (available online).
1.12 Scientific literature:
J. Padua, J.M. Bernardo & M.H. Alves, 2008. Exercicio de intercalibracao em em massas de água fortemente modificadas ? albufeiras no ambito da Directiva-Quadro da Água. Acta do 9º Congresso da Agua, Lisboa. Further information can also be found in Intercalibration Technical Report - Part 2: Lakes.
Goncalves, V., 2007. Contribuicao para o estudo da qualidade ecologica das lagoas dos Acores. Fitoplancton e diatomaceas bentonicas. Dissertacao de doutoramento. Universidade dos Acores. Ponta Delgada. (available online).
1.13 Method developed by: n.a.
Email of developer: n.a.
Institute of developer: n.a.
1.14 Method reported by:
João Ferreira, Maria Margarida de A. L. Correia Raposo de Medeiros
Email of person reporting the method: joao.ferreira@inag.pt, maria.ma.medeiros@azores.gov.pt
Email of institute reporting the method:
Water Institute (Instituto da Água, I.P), Secretaria Regional do Ambiente e do Mar - Direcção Regional do Ordenamento do Território e dos Recursos Hídricos (Environment and the Sea Secretary - Regional Department of Land
1.15 Comments:
Sampling and analysis according to Lake MedGIG defined procedures. Quality evaluation methods for reservoirs are based on available metrics tested and agreed within Lake MedGIG. Assessment method is being tested for Natural Lakes in Azores.

2. Data acquisition

Field sampling/surveying

2.01 Sampling/Survey guidelines:
National sampling protocol: Inag, I.P. (2009). Manual para a Avaliacao da Qualidade Biologica da Agua em Lagos e Albufeiras segundo a Directiva Quadro da Agua - Protocolo de Amostragem e analise para o Fitoplancton. Ministerio do Ambiente, do Ordenamento do Territorio e do Desenvolvimento Regional. Instituto da Agua, I. P. (available online).
2.02 Short description:
Integrated sampling of euphotic zone (2.5 x Secchi depth) with Water Sampler.
2.03 Method to select the sampling/survey site or area: Expert knowledge
2.04 Sampling/survey device: Water sampler
2.05 Specification:
Several water samplers can be used (e.g. Van Dorn, Nasen, Niskin) but automatic Integrating Water Samplers are recommended.
2.06 Sampled/surveyed habitat:
Specification of sampled habitat: Euphotic zone at the deepest site of Lake/Reservoir
Sampled habitat: Single habitat(s)
2.07 Sampled/surveyed zones in areas with tidal influence: not relevant
2.08 Sampling/survey month(s):
1 sample per season (Autumn, Winter, Spring) and 3 samples during Summer (June - September)
2.09 Number of sampling/survey occasions (in time) to classify site or area: 3 samples from the summer period per year
2.10 Number of spatial replicates per sampling/survey occasion to classify site or area: 1 replicate
2.11 Total sampled/surveyed area or volume or total sampling duration to classify site or area:
Variable, depending from euphotic zone. See sample description.

Sample processing

2.12 Minimum size of organisms sampled and processed: n.a.
2.13 Sample treatment:
According to Utermöhl method (CEN Standard EN15204:2006).
Sample is divided (sub-sampling) and organisms of a sub-sample are identified.
2.14 Level of taxonomical identification:
Level: Genus, Species/species groups
Specification of level of determination:
Not depending on the group. Species identification level should be used whenever possible.
2.15 Record of abundance:
Determination of abundance: Individual counts
Abundance is related to: Volume
Unit of the record of abundance: cells/ml
Other relation of abundance: n.a.
Other record of abundance: n.a.
2.16 Quantification of biomass: Chlorophyll-a concentration, Utermöhl technique
2.17 Other biological data: none
2.18 Special cases, exceptions, additions: none
2.19 Comments:

3. Data evaluation

Evaluation

3.01 List of biological metrics:
Chlorophyll-a, Total biovolume, Percentage of Cyanobacteria, Catalan Index (CI) for Northern Reservoirs Type. Chlorophyll-a for Southern Reservoirs Type. For more details see Intercalibration Technical Report - Lakes (Section 2 and 3)
3.02 Does the metric selection differ between types of water bodies: Yes
3.03 Combination rule for multi-metrics: Average metric scores
3.04 From which biological data are the metrics calculated:
List of biological metrics: Aggregated data from multiple sampling/survey occasions in time

Reference conditions

3.05 Scope of reference conditions: Surface water type-specific
3.06 Key source(s) to derive reference conditions:
Scope of reference conditions: Expert knowledge, Historical data, Least Disturbed Conditions
3.07 Reference site characterisation:
Number of sites:
Reference Conditions were derived within MedGIG using sites from all MS. See Intercalibration Technical Report - Part 2: Lakes.
Geographical coverage:
Reference Conditions were derived within MedGIG using sites from all MS. See Intercalibration Technical Report - Part 2: Lakes.
Location of sites: n.a.
Data time period: 2005
Criteria:
For HMWB water bodies true Reference Condition concept doesn?t apply. For these water bodies the ?Reference Conditions? on which status classification is based are within the range of Maximum Ecological Potential (Intercalibration Technical report Part 2: Lakes). Selection of Portuguese ?Reference? Reservoirs for Phytoplankton Biological Quality Element was based on the following pressure variables and thresholds:
- Land Use: less than 20% of the catchment for agricultural land use and the rest remaining as natural or semi-natural coverage;
- Water Level Fluctuation: Historical records show low/medium water level fluctuation (interquartile range less than 20m);
- Cyanobacteria Bloom: Historical absence of cyanobacteria blooms;
- Recreation (including navigation) and Fishing Activities: Low/moderate recreation and fishing activities. Based on expert judgement;
- Total P: Historical records were used in order to make a final selection of ?Reference? reservoirs if they failed to achieve the previous criteria. The threshold of 50 µg/L of Total P was used. Note: variable determined using discrete 0,5 m depth samples.
3.08 Reference community description: Not available yet
3.09 Results expressed as EQR: Yes

Boundary setting

3.10 Setting of ecological status boundaries: Boundaries taken over from the intercalibration exercise
3.11 Boundary setting procedure:
No evident discontinuity was detected on the indexes response to the pressure gradient. Good-Moderate Classes boundary was set as the 95th percentile of the distribution of summer average values for Chlorophyll-a and Total biovolume. Good-Moderate Classes boundary was set as the 90th percentile of the distribution of summer average values for Percentage of Cyanobacteria, Catalan Index (CI).
3.12 "Good status" community: n.a.

Uncertainty

3.13 Consideration of uncertainty: No (to be done)
Specification of uncertainty consideration: n.a.
3.14 Comments:
Assessment Method is still under development in Azores Archipelago, therefore the answers provided in Section C - Data Evaluation only concern the assessment of Reservoirs of Portugal Mainland.

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