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CroTroph-D [id:]

Method: Croatian Diatom Trophic Index [Hrvatski troficki indeks dijatomeja]

1. General information

1.01 GIG: Eastern Continental
Relevant intercalibration types: R-E2, R-E3, R-E4, R-E6, R-EX1, R-EX2, R-EX3, R-EX7, R-EX8
1.02 Category: Rivers
1.03 BQE: Benthic Diatoms
1.04 Country: Croatia
1.05 Specification: none
1.06 Method name: Croatian Diatom Trophic Index
1.07 Original name: Hrvatski troficki indeks dijatomeja
1.08 Status: Method is/will be used in Second RBMP (2015)
1.09 Detected pressure(s):
Catchment land use, Eutrophication, Flow modification, Hydromorphological degradation, Pollution by organic matter, Riparian habitat alteration Pressure-impact-relationship: No, pressure-impact relationship has not been tested.
1.10 Internet reference: n.a.
1.11 Pertinent literature of mandatory character: n.a.
1.12 Scientific literature:
Acs, E., G. Borics, G. Feher, K.T. Kiss, N.M. Rescone, C. Stegner-Kovacs & G. Vabrio, 2009. Implementation of European Water Framework Directive to assessment the water quality of Hungarian running waters with diatoms. Diatomededeligen 33. Ecosurv BQE report phytobenthos ministry of Environment and water, Hungray, October 2005, EuropeAid/114951/D/SV/2002-000-180-04-01-02-02.
Nijboer, R.C., R.K. Johnson, P.F.M. Verdonschot, M. Sommerhäuser & A. Buffagni, 2004. Establishing reference conditions for European streams Hydrobiologia 516: 91-105.
Nijboer, R.C. & A. Schmidt-Kloiber, 2004. The effect of excluding taxa with low abundances or taxa with small distribution ranges on ecological assessment Hydrobiologia 516: 347-363.
Verdonschot, P.F.M. & R.C. Nijboer, 2004.Testing the European stream typology of the Water Framework Directive for macroinvertebrates Hydrobiologia 516: 35-54.
1.13 Method developed by: Andelka Plenkovic-Moraj
Email of developer:
Institute of developer: University of Zagreb, Faculty of Science, Division of Biology
1.14 Method reported by: Dagmar Šurmanovic
Email of person reporting the method:
Email of institute reporting the method: Hrvatske vode
1.15 Comments: none

2. Data acquisition

Field sampling/surveying

2.01 Sampling/Survey guidelines:
Habdiija et al., 2008. Ecological Research of freshwater in Croatia regarding criteria of the Water Framework Directive of EU.
Mihaljevic et al.: Testing of biological methods of ecological status assessment (Water Framework Directive 2000/60/EC) in representative River basins of the Pannonian and Dinaric ecoregions (in preparation)
Instruction Protocol for the ecological Assessment of Running Waters SFOR Implementation of the CE Water Framework Directive: Macrophytes and Phytobenthos (2006).
2.02 Short description:
3 large stones from different river parts, strong tooth brush and scraper, all to one sample bottle and preserving with formaldehyde.
2.03 Method to select the sampling/survey site or area: Expert knowledge
2.04 Sampling/survey device: Brush, Scraper
Other phytobenthos sampling device: direct sampling from soft sediments
Any other sampling device: direct sampling from soft sediments
2.05 Specification: none
2.06 Sampled/surveyed habitat:
Specification of sampled habitat: Hard bottom
Sampled habitat: Single habitat(s)
2.07 Sampled/surveyed zones in areas with tidal influence: not relevant
2.08 Sampling/survey month(s): May to October
2.09 Number of sampling/survey occasions (in time) to classify site or area: Once per year
2.10 Number of spatial replicates per sampling/survey occasion to classify site or area: Minimum 3
2.11 Total sampled/surveyed area or volume or total sampling duration to classify site or area:
3 hard surfaces

Sample processing

2.12 Minimum size of organisms sampled and processed: All diatoms, no matter the size
2.13 Sample treatment:
Diatoms - 400 individuals
Sample is divided (sub-sampling) and organisms of a sub-sample are identified.
2.14 Level of taxonomical identification:
Level: Genus, Species/species groups
Specification of level of determination: n.a.
2.15 Record of abundance:
Determination of abundance: Relative abundance
Abundance is related to: n.a.
Unit of the record of abundance: percentage
2.16 Quantification of biomass: n.a.
2.17 Other biological data: none
2.18 Special cases, exceptions, additions: none
2.19 Comments: none

3. Data evaluation


3.01 List of biological metrics: In preparation (TDICRO and IPS)
3.02 Does the metric selection differ between types of water bodies: No
3.03 Combination rule for multi-metrics: Not relevant
3.04 From which biological data are the metrics calculated:
List of biological metrics: Data from single sampling/survey occasion in time

Reference conditions

3.05 Scope of reference conditions: n.a.
3.06 Key source(s) to derive reference conditions:
Scope of reference conditions:
Existing near-natural reference sites, Expert knowledge, Historical data, Least Disturbed Conditions
3.07 Reference site characterisation:
Number of sites: In preparation
Geographical coverage: In preparation
Location of sites:
Several spring sources, National Park Plitvice Lakes and some others, but in preparation
Data time period: Research project started in 2006
Criteria: According to WFD Ref. Cond. final version
3.08 Reference community description: In preparation
3.09 Results expressed as EQR: n.a.

Boundary setting

3.10 Setting of ecological status boundaries: n.a.
3.11 Boundary setting procedure: Under development
3.12 "Good status" community: Under development


3.13 Consideration of uncertainty: No (to be done)
3.14 Comments: none

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